ABSTRACT
@#Introduction: Risk factors for type 2 diabetes mellitus (T2DM) include obesity and some genetic factors. Obesity involves mild chronic inflammation that predisposes cells to insulin resistance. Two genes that influence obesity and insulin resistance are Proconvertase-1 (PC-1) and resistin (RETN). PC-1 affects the activation of hormones that regulate satiety and hunger. Resistin is one of the inflammatory factors that influence the occurrence of insulin resistance. This study aimed to determine the influence of polymorphism in the PC-1 gene rs1044498 (C>A) and resistin gene RETN + 299 G>A rs3745367 on the risk of diabetes in obese Papua population. Methods: This study involved 58 obese people with T2DM and 58 obese people without DM. We examined the characteristics of blood pressure, lipid profile and insulin resistance by HOMA-IR. The genes examined were PC-1 rs1044498 (C>A) and RETN+ 299 G>A rs3745367 by the PCR-RFLP method. The relationship of gene variations with biochemical parameters was determined with analysis of variance. The results were considered significantly different if P < 0.05. Results: In this study, parameters of diastolic blood pressure, triglycerides and insulin resistance were higher while high density lipoprotein (HDL) levels were lower and significantly different in the obese with T2DM group compared to the obese only group. The carrier of the A allele in the PC-1 gene rs1044498 was higher in the obese group than the obese with T2DM but not significantly different in biochemical parameters. Carrier of the AA genotype in the RETN gene + 299 G>A rs3745367 had higher triglycerides and HOMA-IR and lower HDL levels significantly different (P<0.05) than other genotypes in the obesity with T2DM group. Conclusion: PC-1 rs1044498 gene was a risk factor for obesity but not for T2DM, while RETN gene rs3745367 was a risk factor for dyslipidemia and diabetes in obese people in the Papua population.
ABSTRACT
@#Introduction: The leptin receptor gene (LEPR) variation plays an important role in diseases related with obesity which include Type 2 Diabetes Mellitus (T2DM) and hypertension in some populations. The role of this variation is still controversial and not yet studied in the eastern parts of Indonesia. Hence, this study aimed to explore the correlation of leptin receptor variations (Lys109Arg and Gln223Arg) with blood pressure and blood glucose in T2DM in Ternate population. Methods: This study examined 136 subjects with the age range of 32-76 years old. Five mL of fasting blood were taken to determine blood glucose levels using the GOD-PAP method, and leukocytes were used for genotyping by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) technique methods. Frequencies of genotypes and alleles were analyzed with chi square tests. Correlations of genotypes with the anthropometric measurements were calculated by logistic regression with significance value if p<0.05. Results: Variation of Lys109Arg LEPR gene did not influence the Body Mass Index (BMI), blood pressure, nor blood glucose level. Variation of Gln223Arg LEPR gene also did not influence BMI and blood glucose level, but correlated with blood pressure. Regression analysis after adjusted for age, gender, BMI and blood glucose level showed that this variation remains significantly different. Conclusion: Variation of Gln223Arg LEPR gene correlated with blood pressure but variation of Lys109Arg LEPR gene was not correlated with blood glucose level nor blood pressure. Future study is needed to correlate other genes and examine their effect on metabolic syndrome diseases.
ABSTRACT
@#Background: Interleukin-6 (IL-6) and C-Reactive Protein (CRP) are mediators of inflammatory responses and increase in people who are obese . The increase of IL-6 and CRP levels is modified by polymorphism of -174 G>C IL-6 gene. Aim: The purpose of this study was to investigate the relationship between -174 G>C IL-6 polymorphism gene on the level of IL-6 and CRP in the population of western Indonesia obese who are obese. Methods: In this study, we examined 178 subjects consisting of 89 who are obese with BMI> 25, and controls with BMI between 18.5 and 23. Fasting blood was taken from each subject for the examination of IL-6 and CRP levels by the ELISA method. Determination of genotype -174 G>C IL-6 gene was examined by Polymerase Chain reactionRestriction Fragment Length Polymorphism (PCR-RFLP) methods. Results: The results of this study showed increased levels of IL-6 and CRP in the obese group compared to the controls. In the obese group, CC genotype had higher CRP and lower IL-6 levels than the GC and GG genotypes. The frequency of CC genotype in the obese group was 47.2% compared with 28.1% in controls and this genotype was considered a risk factor for obesity. Carriers of the C genotype as a dominant or a recessive model had greater risk of obesity. Conclusion: It was concluded that the polymorphism - 174G>C IL-6 gene is a risk factor for obesity and is associated with increased levels of IL-6 and CRP in an obese group of the Western Indonesian ethnic population.